The predictable regeneration of intrabony defects often represents a particular challenge for the dental practitioner. Today the combination of Enamel matrix derivative (EMD) with an appropriate flap design represents the gold standard. Meanwhile there is increasing evidence from preclinical and clinical studies, that hyaluronic acid (HA) features a wide range of positive biologic effects on periodontal wound healing and regeneration. Recently, both animal and clinical data have shown the positive effects of HA when used in conjunction with reconstructive periodontal surgery for the regenerative treatment of intrabony defects. The present article explains the surgical procedure for the regeneration of typical intrabony defects with HA based on two clinical cases.